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    • Feature SCaBP phosphorylation is important to SCaBP-PKS regulatory pathway in Arabidopsis.
    Application WB
    Species Arabidopsis thaliana
    Targets SCaBP1
    Catergory Membrane
    Title Phosphorylation of SOS3-like calcium-binding proteins by their interacting SOS2-like protein kinases is a common regulatory mechanism in Arabidopsis.
    Publication Yan Guo, Plant Physiol, 2011
    Methods Anti-phospho-Ser-216 (SCaBP1) polyclonal antibodies were made by AbMart (www.ab-mart.com.cn). Two 8-amino acid peptides (corresponding to amino acids 213–220 of SCaBP1 and 198–205 of CBL1/9) with N-terminal Cys residues, Cys-TFPpSFVFH-NH2 (phosphorylated form) and Cys-TFPSFVFH-NH2 (nonphosphorylated form), were also synthesized by AbMart. The Ser phosphospecific peptide (Cys-TFPpSFVFH-NH2) was used to produce polyclonal phosphospecific antibodies and the nonphosphorylated peptide (Cys-TFPSFVFH-NH2) was used for screening and purification.
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