| Feature | a Large Domesticated Ungulate |
| Application | WB,IF |
| Species | Bos taurus |
| Targets | IgD |
| Catergory | immunoglobulin |
| Title | Expressional analysis of immunoglobulin D in cattle (Bos taurus), a large domesticated ungulate. |
| Publication | Xu B,PLOS ONE, 2012 |
| Methods | anti-bovine IgD mAb 13C2 (Abmart, Shanghai,China) Using a service from the Abmart company (Shanghai, China), the purified proteins and Freund’s adjuvant were used to immunize BALB/c mice.Hybridoma cells were prepared by fusing the splenocytes derived from the immunized mice with Sp2/0 mouse myeloma cells |
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