| Feature | DWARF53 (D53) acts as a repressor of Strigolactones signaling and Strigolactones induce its degradation. |
| Application | Pull Down |
| Species | Oryza sativa |
| Targets | DWARF53(D53)/DWARF14(D14) |
| Catergory | Kinase |
| Title | D14-SCF(D3)-dependent degradation of D53 regulates strigolactone signalling. |
| Publication | Haiyang Wang, Nature, 2013 |
| Methods | The recombination protein was injected into rabbits to produce polyclonal antibodies against D53 (Abmart). For detection of D14 protein, a His-fused polypeptide (amino acids 153–302 of D14) was expressed in BMRosetta (DE3) cells. Purified fusion protein was injected into rats to produce polyclonal antibodies against D14 (Abmart). |
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