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  • Feature Src-mediated PSD-95 phosphorylation activates NMDA receptor.
    Application Blocking
    Species Homo sapiens
    Targets phospho-PSD-95Y523
    Title The Upregulation of NR2A-Containing N-Methyl-D-Aspartate Receptor Function by Tyrosine Phosphorylation of Postsynaptic Density 95 Via Facilitating Src/Proline-Rich Tyrosine Kinase 2 Activation
    Publication Xiao-Yu Hou, Mol Neurobiol, 2014
    Methods A rabbit polyclonal antibodyagainst phospho-PSD-95Y523 (pY523) was raised inNew Zealand white rabbits with a keyhole limpethemocyanin-conjugated pY-containing synthetic peptide(REDSVLSpYETVT) as immunogen, and then purifiedby two-step immunoaffinity chromatography using a columnconjugated to the non-phosphopeptide to eliminateantibodies against nonphosphorylated PSD-95, followedby purification with another column conjugated with theimmunogen (AbMART, Shanghai, China)