Methods |
For western blotting, proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against ACTB (0.19 mg/mL, 42 KDa), TF (0.26 mg/mL, 78.9 KDa), HSP27 (0.47 mg/mL, 22.9 KDa) or VCL (0.23 mg/mL, 116.9 KDa) (Abmart, Inc.). ... |