Reveal a new mechanism for SAMHD1 to control LINE-1 by activating cellular stress granule pathway.
Application
WB,IF
Species
Homo sapiens
Targets
ORF1p
Catergory
Nucleotide binding
Title
SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation
Publication
Hu S, PLoS Genet, 2015
Methods
The purified ORF1p was used to immunize rabbits. Rabbit serum was collected, purified via affinity chromatography (Abmart, Inc) and tested for their recognition of ORF1p in western blotting and immunofluorescence staining.